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Vine Test Lab utilises BIOREBA DAS- ELISA reagents containing complementary antibodies to different isolates of the grapevine leafroll associated closterovirus (GLRaV) type 1 (1,4). All known isolates of GLRav-1 tested so far are detected.

The virus is one out of several closteroviruses associated with leafroll disease occurring on grapevine cultivars grown in Europe, North and South America, Africa, Asia, and Japan (1, 2, 3, 4,)

The concentration of GLRaV-1 in grapevine tissue varies considerably; thus it is important to collect mature leaves (esp. petioles) and bark (phloem) scrapings from dormant canes as close as possible to the head of the vine.

See Fig.1



Vine Test Lab utilises BIOREBA DAS- ELISA reagents containing poly-and monoclonal antibodies which allows for broad-spectrum detection of all scientifically recognised and published isolates of GLRaV-3 to date; notably isolates of genetic variant groups I, II, III, and IV, including recently published serologically distinct isolates in New Zealand (e.g. NZ1, NZ2, NZ1-VM). These broad-spectrum ELISA reagents have been extensively tested with virus collections from New Zealand, South Africa, Switzerland, and USA.

The concentration of GLRaV-3 in grapevine tissue is irregular and distribution of the virus in plants is uneven: it varies according to tissue source and time of year. Well- developed mature leaves especially petioles and veins, from the lower part of the vine late in the growing season as well as bark (phloem) scrapings from mature canes, are the ideal tissue sources.

See fig 1.


Grapevine sample collection takes place:

Leaf samples: (February- April)

Cane samples: (once lignified)